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Miltenyi Biotec cd24
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Cd24, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/bio_rxiv__64898__2026__03__26__711344-263-4-5?v=Miltenyi+Biotec
Average 93 stars, based on 1 article reviews
cd24 - by Bioz Stars, 2026-07
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isotype matched control antibody  (R&D Systems)
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R&D Systems isotype matched control antibody
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/us12613244-531-21-26?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
isotype matched control antibody - by Bioz Stars, 2026-07
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control isotype antibody  (Bio X Cell)
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Bio X Cell control isotype antibody
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Control Isotype Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/pm41931610-271-35-42?v=Bio+X+Cell
Average 97 stars, based on 1 article reviews
control isotype antibody - by Bioz Stars, 2026-07
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antibodies conjugated to coralite488  (Proteintech)
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Proteintech antibodies conjugated to coralite488
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Antibodies Conjugated To Coralite488, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/pm41840717-128-19-25?v=Proteintech
Average 93 stars, based on 1 article reviews
antibodies conjugated to coralite488 - by Bioz Stars, 2026-07
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rat igg2b isotype control antibody 410  (Bio X Cell)
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Bio X Cell rat igg2b isotype control antibody 410
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Rat Igg2b Isotype Control Antibody 410, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/pm41850678-222-25-31?v=Bio+X+Cell
Average 97 stars, based on 1 article reviews
rat igg2b isotype control antibody 410 - by Bioz Stars, 2026-07
97/100 stars
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rat igg2b pe isotype control  (Miltenyi Biotec)
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Miltenyi Biotec rat igg2b pe isotype control
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Rat Igg2b Pe Isotype Control, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg2b pe isotype control - by Bioz Stars, 2026-07
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antibody coralite488  (Proteintech)
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Proteintech antibody coralite488
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Antibody Coralite488, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/pm41770449-76-18-16?v=Proteintech
Average 93 stars, based on 1 article reviews
antibody coralite488 - by Bioz Stars, 2026-07
93/100 stars
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anti igg antibody  (Bio X Cell)
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Bio X Cell anti igg antibody
A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of <t>CD24</t> staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.
Anti Igg Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/pm41771413-90-21-27?v=Bio+X+Cell
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anti igg antibody - by Bioz Stars, 2026-07
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Image Search Results


A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of CD24 staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.

Journal: bioRxiv

Article Title: Acute degron-mediated RUNX1 loss reprograms enhancer activity to epigenetically drive epithelial destabilization and initiate cancer hallmarks

doi: 10.64898/2026.03.26.711344

Figure Lengend Snippet: A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of CD24 staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.

Article Snippet: Cells were stained with CD24 (Miltenyi Biotec, 130-108-352) or isotype (Miltenyi Biotec, 130-124-062) antibodies at the manufacturer’s suggested concentration for 20 minutes at 4°C, washed and acquired using a MACSquant YVB.

Techniques: Binding Assay, Flow Cytometry, Activity Assay, Staining, Control